LOCUS       SRAAFP        874 bp    mRNA            VRT       28-APR-1993
DEFINITION  Sea raven (Hemitripterus americanus) antifreeze polypeptide (AFP)
            mRNA, complete cds.
ACCESSION   J02593
VERSION     J02593.1  GI:213873
KEYWORDS    antifreeze protein.
SOURCE      Sea raven liver, cDNA to mRNA, clone C2-1.
  ORGANISM  Hemitripterus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
            Acanthomorpha; Acanthopterygii; Percomorpha; Scorpaeniformes;
            Cottoidei; Cottidae; Hemitripterus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Ng,N.F., Trinh,K.-Y. and Hew,C.-L.
  TITLE     Structure of an antifreeze polypeptide precursor from the sea
            raven, Hemitripterus americanus
  JOURNAL   J. Biol. Chem. 261, 15690-15695 (1986)
  MEDLINE   87057207
COMMENT     Draft entry and printed copy of the sequence [1] kindly provided by
            N.F.Ng, 11-AUG-1986.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Hemitripterus americanus"
                     /db_xref="taxon:8094"
     sig_peptide     10..96
                     /note="antifreeze polypeptide (AFP) signal peptide"
     CDS             10..597
                     /note="antifreeze polypeptide (AFP) precursor"
                     /codon_start=1
                     /protein_id="AAA49617.1"
                     /db_xref="GI:213874"
                     /translation="MQRQQADTETREDISTAGLSIIFIVCTISTTRMLTVSLLVCAMM
                     ALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYETTAMTWALAETNCM
                     KLGGHLASIHSQEEHSFIQTLNAGVVWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPD
                     DVLAACCMQMTAAADQCWDDLPCPASHKSVCAMTF"
     mat_peptide     97..594
                     /product="antifreeze polypeptide (AFP)"
BASE COUNT      224 a    199 c    215 g    236 t
ORIGIN      154 bp upstream of AluI site.
        1 cacacatgaa tgcagaggca acaggctgac actgaaacaa gagaagatat ttctacagca
       61 gggctatcaa tcatcttcat cgtctgcacc atctctacca cgaggatgct gactgtgtct
      121 ctactggttt gtgccatgat ggctctgact caagctaatg atgacaaaat actcaaaggc
      181 acggctacag aggctggacc ggtctctcag agagccccac caaactgtcc cgctggttgg
      241 caacctcttg gtgaccgctg tatctattat gagacaacag cgatgacttg ggctctggct
      301 gagacaaact gtatgaaatt gggtggacac cttgcatcca tccacagcca ggaggagcat
      361 agtttcattc agaccttgaa tgctggtgtt gtatggatcg gaggctccgc ttgcctccag
      421 gcaggtgctt ggacctggtc tgatggtaca cctatgaatt ttcgttcctg gtgttctacc
      481 aaacctgatg atgtactggc cgcgtgctgt atgcagatga ctgctgcagc tgaccaatgc
      541 tgggatgact tgccttgtcc ggcatcccac aaatcagtct gcgccatgac attctgagct
      601 aacacagagg ccatccatca cacaaacact ttagtgggtg tttgattgtg tgtgttcgca
      661 tactcatctg tgttcgtgtc aacagcctca tgctgaacct gaaggttgaa aatctcatat
      721 gacatcttta attctttgct attgttggag ctgcctgaaa ggatgagacg acaagagctg
      781 gaaagcatct gagggctttt aggaagaaat tgaatggtta tgaaaatgat ggtcttttta
      841 tgtattatgt caaattaaaa ggctgacacg ttga
//
