LOCUS       pBSGUS                  4809 bp    DNA     circular SYN 17-JAN-2000
DEFINITION  pBSGUS - GUS gene on BamHI/SacI fragment from pBI101 cloned into
            pBluescriptKSm13+.
ACCESSION   pBSGUS
KEYWORDS    .
SOURCE      synthetic construct.
  ORGANISM  synthetic construct
            artificial sequence.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Fristensky,B.
  TITLE     Untitled
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Fristensky,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2000) Plant Science, University of Manitoba,
            Winnipeg, MB R3T 2N2, Canada
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="synthetic construct"
     source          1..1831
                     /organism="synthetic construct"
                     /plasmid="pBI101"
                     /note="U12639:2528..4418"
     CDS             24..1832
                     /codon_start=1
                     /transl_table=11
                     /product="beta glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA
                     HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     source          1833..4809
                     /organism="synthetic construct"
                     /plasmid="pBluescriptKSm13+"
                     /note="U52331:complement(653..694)"
BASE COUNT     1200 a   1201 c   1249 g   1159 t
ORIGIN
        1 gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
       61 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
      121 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
      181 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
      241 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
      301 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
      361 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
      421 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
      481 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
      541 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
      601 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
      661 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
      721 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
      781 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
      841 cagtggcagt gaagggccaa cagttcctga ttaaccacaa accgttctac tttactggct
      901 ttggtcgtca tgaagatgcg gacttacgtg gcaaaggatt cgataacgtg ctgatggtgc
      961 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     1021 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     1081 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     1141 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     1201 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     1261 aaccggatac ccgtccgcaa gtgcacggga atatttcgcc actggcggaa gcaacgcgta
     1321 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     1381 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     1441 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     1501 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     1561 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     1621 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     1681 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg
     1741 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     1801 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     1861 cggctacagc ctcgggaatt gctaccgagc tccaattcgc cctatagtga gtcgtattac
     1921 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1981 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2041 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     2101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2521 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3841 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4321 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4381 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4441 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4501 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4561 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4621 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4681 acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
     4741 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
     4801 agcccgggg
//
