Computational Biology Methods (COMP-462) 2016

Computational Biology Methods and Research(COMP-561) 2016

Tuesday and Thursday 8:30-10:00, Macdonald 280


3 credits (462) and 4 credits (561)



Mathieu Blanchette



Trottier 3107



McGill Centre for Bioinformatics



McGill University



Montreal, Quebec, Canada






Telephone: 514-398-5209







Course Abstract:





Computational biology is the sub-discipline of Bioinformatics that is closest in spirit to pure computer science. The main efforts in this field are two-fold. Firstly, we are concerned with creating models for problems from the biosciences (biology, biochemistry, medicine) that are both biologically and mathematically sound. Secondly, we are interested in the design and analysis of efficient, and accurate algorithms that solve these problems in practice and strategies for validation of results.






This course is designed to introduce upper-year undergraduate students and graduate students to this area by examining several classic problems from the field. The intention of the course is to act as a gateway whereby, upon completion of the course, students will have the necessary biology, mathematics and computer science background to attend graduate level courses in bioinformatics geared towards specific topics (phylogenetics, genomic evolution, functional genomics, proteomics). The course is designed in such a manner that no previous formal training in biology is required of the students.






The necessary mathematical background consists of the lower level discrete structures and probablity courses, since topics such as maximum likelihood estimation, hidden Markov models, and dynamic programming will be used repeatedly throughout the material. (Both maximum likelihood and hidden Markov models will be introduced at a basic level however.) Students will be required to have already taken the lower level algorithms/data-structures, numerical computing and theoretical computer science courses.







COMP 251 Data Structures and Algorithms



MATH 323 Probability Theory (or equivalent)







Important Note for undergraduate students: If a student does not have the prerequisities for this course, the Faculty of Science will delete this course from their record.




Whereas, McGill University values academic integrity;

Whereas, every term, there are new students who register for the first time at McGill and who need to be informed about academic integrity;

Whereas, it is beneficial to remind returning students about academic integrity;

Be it resolved that instructors include the following statement on all course outlines: McGill University values academic integrity. Therefore all students must understand the meaning and consequences of cheating, plagiarism and other academic offences under the Code of Student Conduct and Disciplinary Procedures (see for more information).

Be it further resolved that failure by an instructor to include a statement about academic integrity on a course outline shall not constitute an excuse by a student for violating the Code of Student Conduct and Disciplinary Procedures.



Office Hours:





M. Blanchette: Tuesday 10:00-11:30, in Trottier 3107

Jeremy George Filteau: Th 10:00-11:30, in Trottier 3090. jeremy.georges-filteau AT

Mohammed Ali Atiia: Fr 15:30 -17:00, in Trottier 3090. atiia AT


Book and Material:





Bernhard Haubold and Thomas Wiehe. Introduction to Computational Biology: An Evolutionary Approach , Burkhauser Basel, 2007




Probably the best introductory book out there. Its level is ideal for the course, but it does not go much beyond this.






Durbin, Eddy, Krogh, Michinson, Biological Sequence Analysis, Cambridge, 1998.






Also not required, this book is particularly good for learning some of the basics of statistical inference/machine learning.






Jones, N.C. and Pevzner, P. An Introduction to Bioinformatics Algorithms, MIT press, 2004



You are not required to buy this book, however it is a good book for understanding some of the classic problems in computational biology and the algorithms used to solve these problems.






Campbell, A. M., and Heyer, L.J. Discovering genomics, proteomics, and bioinformatics, Benjamin Cummings, 2002



Also not required, this is a good primary for computer scientists that covers the basics of genomics, genetics, and proteomics.






Alberts, Johnson, Lewis, Raff, Roberts, Walter Molecular Biology of the Cell, Garland, 2002



This is a widely used and comprehensive book covering the biology of the cell. It is a good place to start when you want to explore a new topic.


Evaluation for COMP 462:




4 assignments

40% total (10% each)


Midterm, October 12, in classth



Final Exam, during exam period



Evaluation for COMP 561:




3 assignments (the first three)

30% total (10% each)





Midterm, October 12th



Final Exam, during exam period



Computer Science/mathematics topics:





Basic probability and statistics (ubiquitous)



Dynamic programming (sequence alignment)



Approximation algorithms (string alignment)



Advanced data structures (suffix trees)



Numerical techniques (least squares fits)



Experimental design





Concepts from biology and biotechnologies





Models of evolution



Sequence comparison






Gene expression and regulation



Peptide identification



RNA secondary structure



DNA sequencing



Population genetics


Course outline


Lecture 1,2: Introduction to molecular biology and genomics.





Topics: Basic Questions, Basic Strategies, Introduction to molecular biology and genomics.



Background Reading: Chapter 1 of Artificial Intelligence and Molecular Biology , by L. Hunter



On-line Resources: Lecture notes by Dudoit and Gentleman


Lecture 3-7: Sequence evolution and sequence alignment.





Topics: Introduction to sequence evolution. Global and local alignment; Gapping; BLAST algorithm; Multiple Alignments.



Background Reading: Chapter 6 of Jones, Pevzner; Chapter 6.2 of Ewans, Grant.



Math/Algorithms: Dynamic Programming



Applications: Gene finding.



On-line Resources:



Additional material for COMP 561: Chapter 6 of Durbin and Eddy.



Lecture 8-11: Evolutionary models and phylogenetic Tree construction.





Topics: Discrete and continuous nucleotide and amino acid substitution models Distance-based methods; Parsimony; Maximum Likelihood.



Background Reading: Chapters 7-8 of Durbin et al. or Chapter 8 and 9.1-9.5 of Haubold and Wiehe.



Math/Algorithms: Discrete algorithm design; Maximum likelihood.



On-line Resources:



Midterm exam, October 12, in class (open book).







Lecture 13-16: Hidden Markov Models.





Topics: Forward, backward, Viterbi, Baum-Welch algorithms. Gene-finding HMMs. Profile HMMs.



Background Reading: Chapters 3 and 5 of Durbin et al.



Math/Algorithms: Markov processes; Dynamic programming; Parameter estimation.



Application: Gene finding



On-line Resources:


Lecture 17-18: Motif discovery.





Topics: Modelling and searching for signals in DNA..



Background Reading: Chapter 5 of Ewans, Grant; Chapter 4 of Jones, Pevzner



Math/Algorithms: Probability theory; Markov processes; exhaustive search; Gibb's sampling (intro)



Applications: Searching for repeats. Identifying transcription factor binding sites.



On-line Resources:




Lecture 19-20: Gene Expression Analysis.





Topics: Class distinction; Class prediction; Class discovery.



Background Reading: Chapter 10 of Jones, Pevzner.



Math/Algorithms: Differential expression; Principal Component Analysis; Clustering; Graph theory.



On-line Resources:


Lecture 21-22: Sequence assembly and Protein Identification.





Topics: DNA sequencing; Sequence assembly problem; Peptide identification; Peptide sequencing; Protein Identification.



Background Reading: Chapter 8.10-8.15 of Jones, Pevzner.



Math/Algorithms: Graph theory; Dynamic programming..



On-line Resources:



Lecture 23,24: Introduction to population genetics





Topics: Polymorphisms, haplotypes. Genotyping by sequencing. Phasing.



Background Reading: TBD





Final exam, during the exam session.





Mathieu Blanchette (based on Mike Hallett's syllabus for COMP 462)